Journal: Stem cells and development
Article Title: Study on the Safety of Human Oligodendrocyte Precursor Cell Transplantation in Young Animals and Its Efficacy on Myelination.
doi: 10.1089/scd.2021.0012
Figure Lengend Snippet: FIG. 5. Immunological evaluation of chronic toxicity experiment. Effects of the transplanted cells on the immune system of the rats were evaluated. (A, B) Immune cell detection in peripheral blood. Indicators include CD3+CD4+ and CD3+CD8+. (C, D) Detection of spleen immune cells. Indicators include CD3, CD4, and CD8+. (E) Detection of cytokines in serum. Indicators include IFN-g, TNF-a, IL-2, IL-4, IL-5, and IL-6. Bars represent means. Error bars show the standard error of the mean. IL, interleukin; TNF-a, tumor necrosis factor alpha.
Article Snippet: These two cell suspensions were surface stained with CD3 APC mouse anti-human (Cat. #562799; BD Biosciences), CD4 APC-A750 (Cat. #130-093-581; Miltenyi Biotec), CD8 PC5.5 (Cat. #FAB2585A; R&D Systems), and CD45RA PC7 mouse anti-human antibodies for flow cytometry (FACSanto II; BD Biosciences).
Techniques: